Macrolide Resistance Conferred by Base Substitutions in 23S rRNA

Birte Vester and Stephen Douthwaite Substitutions in 23 S rRNA Macrolide Resistance Conferred

Macrolide Resistance–associated 23S rRNA Mutation in Mycoplasma genitalium, Japan

because we included samples from children hospitalized with or without respiratory disease. BLAST search (www.ncbi.nlm. nih.gov/blast/Blast.cgi) and phylogenetic analysis of amplicons from PCR and nested PCR indicated that samples were positive for HKU1 genotype B (samples BRA169, BRA90, and BRA99) or HKU1 genotype A (samples BRA09, BRA37, and BRA104). The sequences obtained in this study have ...

Resistance phenotypes conferred by macrolide phosphotransferases.

This study aims to compare the resistance phenotypes conferred by various genes encoding enzymes that phosphorylate erythromycin. The mph genes were cloned into Escherichia coli AG100A susceptible to macrolides and ketolides following disruption of the AcrAB pump. An 882 bp sequence containing a premature stop codon, homologous to the three other previously described mph genes and present widel...

Macrolide resistance conferred by rRNA mutations in field isolates of Mannheimia haemolytica and Pasteurella multocida.

OBJECTIVES To determine how resistance to macrolides is conferred in field isolates of Pasteurella multocida and Mannheimia haemolytica that lack previously identified resistance determinants for rRNA methylation, efflux and macrolide-modifying enzymes. METHODS Isolates of P. multocida and M. haemolytica identified as being highly resistant (MICs >64 mg/L) to the macrolides erythromycin, gami...

23S rRNA base pair 2057-2611 determines ketolide susceptibility and fitness cost of the macrolide resistance mutation 2058A-->G.

The 23S rRNA A2058G alteration mediates macrolide, lincosamide, and streptogramin B resistance in the bacterial domain and determines the selectivity of macrolide antibiotics for eubacterial ribosomes, as opposed to eukaryotic ribosomes. However, this mutation is associated with a disparate resistance phenotype: It confers high-level resistance to ketolides in mycobacteria but only marginally a...